Seminaire CIRI: Benoit LACOMBE

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Date / Heure
Date(s) - 23/01/2017
14 h 30 min - 15 h 30 min

salle des Thèses Chantal Rabourdin-Combes

Benoit LACOMBE (Institut Cochin, Paris. INSERM , U1016/CNRS UMR 8104. Team : Retroviruses, Quiescence and Proliferation (Equipe : Dr C. Ramirez).);

Titre:  » Molecular characterization of a new Tat-dependent transcription inhibitor, the Spironolactone ”.
Résumé: Spironolactone (SP) is an aldosterone antagonist used in hypertension treatment for many years. Jurkat and primary CD4 + T cells infected by HIV-1 or HIV-2 and treated by SP show a strong degradation of the XPB protein and an important inhibition of viral infection. To confirm specificity of SP action on HIV, we have used a structural analogue of SP, Eplerenone (EPL), which can’t degrade XPB. On the contrary, EPL is unable to inhibit infection of cells previously infected by HIV. Otherwise, SP inhibition of HIV seems to be specific of Tat dependent transcription from the HIV promoter, because activation of this promoter with PMA or TNF is not affected by SP. Quantification of viral and cellular RNA show that only LTR transcription is affected by SP. Finally, SP is also able to inhibit HIV LTR reactivation from latently infected T cells. This drug appears as a new anti-HIV compound and gives new perspectives concerning transcriptional inhibitors of HIV replication.  »

Contact: Marlène Dreux