Séminaire CIRI: Dr. Stacy HORNER

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Date / Heure
Date(s) - 10/02/2020
9 h 00 min - 10 h 00 min

Emplacement
salle des Thèses Chantal Rabourdin-Combes


Dr. Stacy HORNER (Lab Head at Duke University Medical Center Durham, NC – USA): « A role for the RNA modification m6A at the virus-host interface »

Résumé:
The RNA modification N6-methyladenosine (m6A) plays an important role in the posttranscriptional control of eukaryotic mRNA fate and dynamically modulates important biological processes. We have previously found that m6A modification of viral RNA genomes regulates infection by RNA viruses in the Flaviviridae family. However, the function of epitranscriptomic m6A modification of cellular mRNA during viral infection is still unclear. Our work now reveals that infection by the Flaviviridae members dengue virus (DENV), Zika virus (ZIKV), West Nile virus (WNV), and hepatitis C virus (HCV) similarly alters the m6A modification of a set of specific cellular transcripts without affecting the overall distribution of m6A across the epitranscriptome. We find that some of the observed changes in m6A modification are a result of innate immune sensing and ER stress, cellular pathways that are activated by Flaviviridae infection, suggesting that signal transduction from these pathways influences the specificity of the m6A methyltransferase complex. We then further elucidate how m6A regulates expression of these specific transcripts during Flaviviridae infection and how post-transcriptional control of these transcripts by m6A impacts viral infection. Taken together, these observations provide insights into how m6A modification of cellular transcripts is altered during viral infection and how this influences the outcome of Flaviviridae infection.

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