Séminaire CIRI: Pr. Thomas PIETSCHMANN
Date / Heure
Date(s) - 31/05/2018
11 h 00 min - 12 h 00 min
salle des Thèses Chantal Rabourdin-Combes
Pr. Thomas PIETSCHMANN (TWINCORE – Zentrum für Experimentelle und Klinische Infektionsforschung GmbH. Hannover, Germany): “Towards animal models for HCV vaccine research: Determinants of HCV species tropism”
HCV infection affects ca. 71 million individuals worldwide. These patients are at risk to develop severe liver disease including cirrhosis and liver cancer. Novel therapies have been licensed and HCV infection can be cured. However, these therapies are expensive, many HCV infected patients are not diagnosed, and treatment-induced viral clearance does not protect from HCV re-infection. Therefore, development of a prophylactic vaccine is an important clinical need. Unfortunately, immunocompetent animal models are lacking so that analysis of protective immunity against HCV, and prioritization of vaccine candidates is difficult. HCV has a narrow species tropism and naturally infects only humans. The viral and host determinants governing HCV species tropism are incompletely defined. We used genome-wide cDNA screens and iterative virus selection and adaptation approaches to dissect viral and host factors that define the mechanisms of HCV species tropism. Using these approaches we identified two mouse factors constitutively expressed in mouse livers that restrict HCV infection. Moreover, viral variants with extended species tropism were developed and characterized. Ultimately, this work should facilitate development of animal models for HCV vaccine research.
contact : François-Loïc Cosset