Interaction hôte-pathogène lors de l’infection lentivirale

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ISG20, a new sentinel of translation

A new mechanism of translational control of foreign genetic elements orchestrated by the Interferon-Sensitive Gene 20 (ISG20) has been identified by the laboratory in a recent study accepted on PlosPathogens. ISG20 was previously known to inhibit a large panel of viruses but the underlaying mechanism of inhibition was thought to be the complete degradation of the target RNA. Our recent study indicates that ISG20 is able to block the translation of viral, but also of ectopically expressed genetic material, in the absence of ouvert degradation of its RNA target. This mechanism of translational control seems able to discriminate between RNAs derived from chromosomal genes (self) and foreign genetic elements independently of their viral/non viral origins.

This study thus reveals the existence of a novel mechanism of regulation with which the cell is able to distinguish its own genetic material from the one of invading elements.

This work has been carried out via a collaboration with the CIRI groups Ohlmann, Mahieux and Gruffat, as well as with the DU’s laboratory at the ECNU in Shanghai, China.

Picture: Cells either infected with a VSV-virus bearing a GFP reporter, or directly transfected with a GFP-coding plasmid. The structure of ISG20 was determined in Horio et al, FEBS let. 2004. Residues that inactivate or not the protein’s functions have been colored in violet and yellow, respectively.


Recently accepted in Plos Pathogens:

The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation.
Nannan Wu, Xuan-Nhi Nguyen, Li Wang, Romain Appourchaux, Chengfei Zhang, Baptiste Panthu, Henri Gruffat, Chloé Journo, Sandrine Alais, Juliang Qin, Na Zhang, Kevin Tartour, Frédéric Catez, Renaud Mahieux, Theophile Ohlmann, Mingyao Liu, Bing Du, Andrea Cimarelli.  Plos Pathogens: October 10, 2019